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Detection of mitochondrial fission with orientation‐dependent optical Fourier filters
Author(s) -
Pasternack Robert M.,
Zheng JingYi,
Boustany Nada N.
Publication year - 2011
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.21011
Subject(s) - orientation (vector space) , fourier transform , fission , mitochondrial fission , fourier analysis , biophysics , physics , biological system , biology , microbiology and biotechnology , mitochondrion , nuclear physics , mathematics , geometry , quantum mechanics , neutron
We utilize a recently developed optical imaging method based on Fourier processing with Gabor‐like filters to detect changes in light scattering resulting from alterations in mitochondrial structure in endothelial cells undergoing apoptosis. Imaging based on Gabor filters shows a significant decrease in the orientation of subcellular organelles at 60 to 100 minutes following apoptosis induction and concomitant with mitochondrial fragmentation observed by fluorescence. The optical scatter changes can be detected at low resolution at the whole cell level. At high resolution, we combine fluorescence imaging of the mitochondria with optical Fourier‐based imaging to demonstrate that the dynamic decrease in organelle orientation measured by optical Gabor filtering is spatially associated with fluorescent mitochondria and remains largely absent from nonfluorescent subcellular regions. These results provide strong evidence that the optical Gabor responses track mitochondrial fission during apoptosis and can be used to provide label‐free, rapid monitoring of this morphological process within single cells. © 2011 International Society for Advancement of Cytometry