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Microfluidic impedance‐based flow cytometry
Author(s) -
Cheung Karen C.,
Di Berardino Marco,
SchadeKampmann Grit,
Hebeisen Monika,
Pierzchalski Arkadiusz,
Bocsi Jozsef,
Mittag Anja,
Tárnok Attila
Publication year - 2010
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20910
Subject(s) - microfluidics , microscale chemistry , software portability , electrical impedance , computer science , cytometry , nanotechnology , materials science , biological system , flow cytometry , electrical engineering , engineering , biology , mathematics education , mathematics , genetics , programming language
Microfabricated flow cytometers can detect, count, and analyze cells or particles using microfluidics and electronics to give impedance‐based characterization. Such systems are being developed to provide simple, low‐cost, label‐free, and portable solutions for cell analysis. Recent work using microfabricated systems has demonstrated the capability to analyze micro‐organisms, erythrocytes, leukocytes, and animal and human cell lines. Multifrequency impedance measurements can give multiparametric, high‐content data that can be used to distinguish cell types. New combinations of microfluidic sample handling design and microscale flow phenomena have been used to focus and position cells within the channel for improved sensitivity. Robust designs will enable focusing at high flowrates while reducing requirements for control over multiple sample and sheath flows. Although microfluidic impedance‐based flow cytometers have not yet or may never reach the extremely high throughput of conventional flow cytometers, the advantages of portability, simplicity, and ability to analyze single cells in small populations are, nevertheless, where chip‐based cytometry can make a large impact. © 2010 International Society for Advancement of Cytometry