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Recognition of Naegleriae ameba surface protein epitopes by anti‐human CD45 antibodies
Author(s) -
Ravine Terrence J.,
Polski Jacek M.,
Jenkins James
Publication year - 2010
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20865
Subject(s) - epitope , monoclonal antibody , biology , antibody , phagocytosis , immunohistochemistry , microbiology and biotechnology , naegleria fowleri , cluster of differentiation , naegleria , protozoa , virology , immunology , cell , biochemistry , meningoencephalitis
Phagocytosis is a highly conserved mechanism exhibited by both free‐living amebas and mammalian blood cells. Similarities demonstrated by either cell type during engulfment of the same bacterial species may imply analogous surface proteins involved in receptor‐mediated endocytosis. The increased availability of anti‐human leukocyte antibodies or clusters of differentiation (CD) markers used in conjunction with flow cytometric (FCM) and/or immunohistochemical (IHC) analysis provides investigators with a relatively easy method to screen different cell populations for comparable plasma membrane proteins. In this study, we incubated Naegleria and Acanthamoeba amebas with several directly conjugated anti‐human leukocyte monoclonal antibodies (mAb) for similarly recognized amebic epitopes. CD marker selection was based upon a recognized role of each mAb in phagocyte activation and/or uptake of bacteria. These included CD14, CD45, and CD206. In FCM, only one CD45 antibody demonstrated strong reactivity with both Naegleria fowleri and Naegleria gruberi that was not expressed in similarly tested Acanthamoeba species. Additional testing of N. gruberi by IHC demonstrated reactivity to a different CD45 antibody. Our results suggest a possible utility of using anti‐human leukocyte antibodies to screen amebic cells for similarly expressed protein epitopes. In doing so, several important items must be considered when selecting potential mAbs for testing to increase the probability of a positive result. © 2010 International Society for Advancement of Cytometry