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Simultaneous characterization of progenitor cell compartments in adult human liver
Author(s) -
Porretti Laura,
Cattaneo Alessandra,
Colombo Federico,
Lopa Raffaella,
Rossi Giorgio,
Mazzaferro Vincenzo,
Battiston Carlo,
SvegliatiBaroni Gianluca,
Bertolini Francesco,
Rebulla Paolo,
Prati Daniele
Publication year - 2010
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20834
Subject(s) - progenitor cell , cd34 , mesenchymal stem cell , biology , stem cell , epithelial cell adhesion molecule , haematopoiesis , endothelial stem cell , cd90 , microbiology and biotechnology , cd146 , flow cytometry , pathology , cancer research , immunology , cell adhesion molecule , medicine , in vitro , biochemistry
The human liver is a complex tissue consisting of epithelial, endothelial, hematopoietic, and mesenchymal elements that probably derive from multiple lineage‐committed progenitors, but no comprehensive study aimed at identifying and characterizing intrahepatic precursors has yet been published. Cell suspensions for this study were obtained by enzymatic digestion of liver specimens taken from 20 patients with chronic liver disease and 13 multiorgan donors. Stem and progenitor cells were first isolated, amplified, and characterized ex vivo according to previously validated methods, and then optimized flow cytometry was used to assess their relative frequencies and characterize their immunophenotypes in the clinical specimens. Stem and progenitor cells committed to hematopoietic, endothelial, epithelial, and mesenchymal lineages were clearly identifiable in livers from both healthy and diseased subjects. Within the mononuclear liver cell compartment, epithelial progenitors [epithelial cell adhesion molecule (EpCAM) + /CD49f + /CD29 + /CD45 − ] accounted for 2.7–3.5% whereas hematopoietic (CD34 + /CD45 + ), endothelial [vascular endothelial growth factor‐2 (KDR) + /CD146 + /CD45 − ], and mesenchymal [CD73 + /CD105 + /CD90 (Thy‐1) + /CD45 − ] stem cells and progenitors accounted for smaller fractions (0.02–0.6%). The patients' livers had higher percentages of hematopoietic and endothelial precursors than those of the donors. In conclusion, we identified and characterized precursors committed to four different lineages in adult human liver. We also optimized a flow cytometry approach that will be useful in exploring the contribution of these cells to the pathogenesis of liver disease. © 2009 International Society for Advancement of Cytometry