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Differentiation of alloreactive versus CD3/CD28 stimulated T‐lymphocytes using Raman spectroscopy: A greater specificity for noninvasive acute renal allograft rejection detection
Author(s) -
Brown Kristian L.,
Palyvoda Olena Y.,
Thakur Jagdish S.,
NehlsenCannarella Sandra L.,
Fagoaga Omar R.,
Gruber Scott A.,
Auner Gregory W.
Publication year - 2009
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20797
Subject(s) - cd3 , cd28 , raman spectroscopy , transplantation , flow cytometry , t cell , immunology , microbiology and biotechnology , cd8 , chemistry , medicine , antigen , biology , immune system , physics , optics
Abstract Acute rejection (AR) remains problematic in renal transplantation. As a marker, serum creatinine is limited, warranting a more effective screening tool. Raman spectroscopy (RS) can detect T‐cell activation with high sensitivity. In this study we explore its specificity. Seventy‐five inactivated, 40 alloantigen‐activated, and 75 CD3/CD28‐activated T cells were analyzed using RS. CD3/CD28‐activated peak magnitudes (PM) were 4.3% to 23.9% lower than inactivated PM at positions: 903, 1031, 1069, 1093, 1155, 1326, and 1449 cm −1 , with a difference in peak ratio (PR) observed at the 1182:1195 cm −1 position (0.91 ± 0.06 vs. 1.2 ± 0.01, respectively: P = 0.006). Differences in CD3/CD28‐ and alloantigen‐activated PM were observed at: 903, 1031, 1093, 1155, 1326, and 1449 cm −1 , with no PR differences at the 1182:1195 cm −1 position (0.91 ± 0.06 vs. 0.86 ± 0.09: P = 0.8). Spectral signature separation of CD3/CD28—and alloantigen‐activated groups was 100% specific and sensitive. We conclude that RS can differentiate T cells activated by different stimuli with high sensitivity and specificity. © 2009 International Society for Advancement of Cytometry