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Flow cytometric investigation of neutrophil oxidative burst and apoptosis in physiological and pathological situations
Author(s) -
Elbim Carole,
Lizard Gérard
Publication year - 2009
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20726
Subject(s) - respiratory burst , flow cytometry , apoptosis , neutrophil extracellular traps , oxidative phosphorylation , pathological , oxidative stress , microbiology and biotechnology , biology , chemistry , inflammation , pathology , immunology , medicine , biochemistry
Flow cytometric analysis provides a rapid screen for abnormalities of polymorphonuclear neutrophils (PMN) function and reflect their behavior in vivo more accurately. This review summarizes the major fluorescent probes used to study PMN oxidative burst and apoptosis using flow cytometry (FCM). We also provide examples of FCM studies in physiological and pathological situations, illustrating the advantages of FCM for assessment of PMN oxidative burst and PMN apoptosis. These data point to the role of FCM in detecting primary immunodeficiencies such as IRAK4 deficiency and support the use of the assessment of the PMN oxidative burst for routine testing in patients with bacterial infections. We also demonstrate the utility of whole‐blood analysis using FCM for a better understanding of PMN functionality, i.e., tuning PMN responses to inflammatory stimuli. Finally, FCM permits a simultaneous analysis of phenotypic, functional and morphometric parameters assessing whole‐blood PMN apoptosis, in particular in response to Toll‐like receptor agonists and during simian immunodeficiency virus infection. © 2009 International Society for Advancement of Cytometry