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MIFlowCyt: The minimum information about a flow cytometry experiment
Author(s) -
Lee Jamie A.,
Spidlen Josef,
Boyce Keith,
Cai Jennifer,
Crosbie Nicholas,
Dalphin Mark,
Furlong Jeff,
Gasparetto Maura,
Goldberg Michael,
Goralczyk Elizabeth M.,
Hyun Bill,
Jansen Kirstin,
Kollmann Tobias,
Kong Megan,
Leif Robert,
McWeeney Shan,
Moloshok Thomas D.,
Moore Wayne,
Nolan Garry,
Nolan John,
NikolichZugich Janko,
Parrish David,
Purcell Barclay,
Qian Yu,
Selvaraj Biruntha,
Smith Clayton,
Tchuvatkina Olga,
Wertheimer Anne,
Wilkinson Peter,
Wilson Christopher,
Wood James,
Zigon Robert,
Scheuermann Richard H.,
Brinkman Ryan R.
Publication year - 2008
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20623
Subject(s) - computer science , software , reuse , data science , data flow diagram , publishing , standardization , information retrieval , database , biology , ecology , political science , law , programming language , operating system
A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross‐disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoptionof MIFlowCyt by the scientific and publishing communities will facilitate third‐party understanding and reuse of FCM data. © 2008 International Society for Advancement of Cytometry