Use of CD137 to study the full repertoire of CD8+ T cells without the need to know epitope specificities | Zendy

Wölfl Matthias | Zendy; Kuball Jürgen | Zendy; Eyrich Matthias | Zendy; Schlegel Paul G. | Zendy; Greenberg Philip D. | Zendy

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Use of CD137 to study the full repertoire of CD8+ T cells without the need to know epitope specificities

Author(s) -

Wölfl Matthias,

Kuball Jürgen,

Eyrich Matthias,

Schlegel Paul G.,

Greenberg Philip D.

Publication year - 2008

Publication title -

cytometry part a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.316

H-Index - 90

eISSN - 1552-4930

pISSN - 1552-4922

DOI - 10.1002/cyto.a.20594

Subject(s) - cd137 , antigen , epitope , cd8 , computational biology , biology , t cell receptor , function (biology) , repertoire , phenotype , microbiology and biotechnology , immunology , t cell , immune system , genetics , physics , gene , acoustics

CD137 (4‐1BB) is a member of the TNFR‐family with costimulatory function, triggering prosurvival signals in activated T‐cells. Upregulation of CD137 upon stimulation allows identifying and isolating live, human antigen‐specific CD8+ T‐cells of all phenotypes, and therefore provides a comprehensive detection method. Furthermore responses against antigen mixtures can be easily detected, enabling antigen discovery in a stepwise deconvoluting approach. In this article, we will discuss various aspects of this methodology, including potential pitfalls as well as a variety of applications, as illustrated by examples from our laboratory. © 2008 International Society for Advancement of Cytometry

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