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Broadening cell selection criteria with micropallet arrays of adherent cells
Author(s) -
Wang Yuli,
Young Grace,
Aoto Phillip C.,
Pai JengHao,
Bachman Mark,
Li G. P.,
Sims Christopher E.,
Allbritton Nancy L.
Publication year - 2007
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20424
Subject(s) - immunolabeling , cell sorting , pallet , population , cell , sorting , selection (genetic algorithm) , fluorescence , biology , microbiology and biotechnology , biological system , computational biology , computer science , immunology , genetics , artificial intelligence , physics , algorithm , engineering , immunohistochemistry , medicine , environmental health , quantum mechanics , mechanical engineering
Background: A host of technologies exists for the separation of living, nonadherent cells, with separation decisions typically based on fluorescence or immunolabeling of cells. Methods to separate adherent cells as well as to broaden the range of possible sorting criteria would be of high value and complementary to existing strategies. Methods: Cells were cultured on arrays of releasable pallets. The arrays were screened and individual cell(s)/pallets were released and collected. Results: Conventional fluorescence and immunolabeling of cells were compatible with the pallet arrays, as were separations based on gene expression. By varying the size of the pallet and the number of cells cultured on the array, single cells or clonal colonies of cells were isolated from a heterogeneous population. Since cells remained adherent throughout the isolation process, separations based on morphologic characteristics, for example cell shape, were feasible. Repeated measurements of each cell in an array were performed permitting the selection of cells based on their temporal behavior, e.g. growth rate. Conclusions: The pallet array system provides the flexibility to select and collect adherent cells based on phenotypic and temporal criteria and other characteristics not accessible by alternative methods. © 2007 International Society for Analytical Cytology