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Automated laser scanning cytometry: A powerful tool for multi‐parameter analysis of drug‐induced apoptosis
Author(s) -
Holme Andrea Lisa,
Yadav Sanjiv Kumar,
Pervaiz Shazib
Publication year - 2007
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20362
Subject(s) - flow cytometry , cytometry , etoposide , single cell analysis , apoptosis , population , drug , camptothecin , biology , cell , pharmacology , microbiology and biotechnology , biochemistry , chemotherapy , medicine , genetics , environmental health
Background: Simultaneous analysis of multiple intracellular events is critical for assessing the effect of biological response modifiers, including the efficacy of chemotherapy. Here we used the automated laser scanning cytometry (LSC) for multi‐parameter analysis of drug‐induced tumor cell apoptosis. Materials: Using 2‐mercaptopyridine‐ N ‐oxide‐hydrate sodium salt, or the commonly used chemotherapeutic agents etoposide and camptothecin, we performed simultaneous analyses of apoptosis‐related morphological features as well as fluorescence‐based biochemical changes in a 96‐well format. Results: We demonstrate the scope of LSC as a platform for comparing multiple variables between different cell populations, distinguishing unique events at a single cell level within a sample population, and enabling simultaneous screenings in a single assay at multiple dosages and time‐points. Conclusion: These data underscore the power of LSC for simultaneous multi‐parameter analysis, which could have implications for screening or assessing the efficacy of drug responses in heterogeneous cell populations and at the single cell level. © 2007 International Society for Analytical Cytology.