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Flow cytometry controls, instrument setup, and the determination of positivity
Author(s) -
Maecker Holden T.,
Trotter Joseph
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20333
Subject(s) - flow cytometry , flow (mathematics) , cytometry , stopped flow , chemistry , chromatography , medicine , mechanics , physics , immunology , quantum mechanics , reaction rate constant , kinetics
A frequent goal of flow cytometric analysis is to classify cells as positive or negative for a given marker, or to determine the precise ratio of positive to negative cells. This requires good and reproducible instrument setup, and careful use of controls for analyzing and interpreting the data. The type of controls to include in various kinds of flow cytometry experiments is a matter of some debate and discussion. In this tutorial, we classify controls in various categories, describe the options within each category, and discuss the merits of each option. © 2006 International Society for Analytical Cytology