Premium
Hyperspectral imaging system using acousto‐optic tunable filter for flow cytometry applications
Author(s) -
Kasili Paul M.,
VoDinh Tuan
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20307
Subject(s) - hyperspectral imaging , flow cytometry , cytometry , filter (signal processing) , optical filter , chemical imaging , spectral imaging , materials science , remote sensing , computer science , optics , optoelectronics , artificial intelligence , computer vision , physics , biology , microbiology and biotechnology , geology
A major advantage of flow cytometry is its flexible and open instrument configuration, which is highly suitable for systems integration. This flexibility permits the coupling of auxiliary instrumentation that may offer the measurement of parameters other than those typically measured by this multiparameter measurement technique. On the basis of this advantage, we explore the principle and application of hyperspectral imaging (HSI), which has the potential to be a useful add‐on feature to flow cytometry applications. Application of HSI to flow cytometry involves the acquisition of spatial information and rendering it in spectral form. In this work, we describe the development and application of an HSI system which provides both spectral and spatial information. Spectral information was generated by obtaining an entire spectrum of a single sample site within a wavelength region of interest, while spatial information was generated by recording a two‐dimensional (2D) image of an area of the sample of interest at one specific wavelength. HSI is a promising additional feature to flow cytometry since it can provide both spatial (image format) and spectral information in addition to the multiparameter information already available from flow cytometry measurements. © 2006 International Society for Analytical Cytology