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Phenotypic comparison of multiple monocyte‐related populations in murine peripheral blood and bone marrow
Author(s) -
Schlueter Annette J.,
Glasgow Judith K.
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20262
Subject(s) - monocyte , biology , phenotype , bone marrow , flow cytometry , immunology , population , chemokine receptor , myeloid , chemokine , immunophenotyping , receptor , pathology , inflammation , medicine , genetics , gene , environmental health
Background: Monocyte subsets are not well defined in murine peripheral blood (PB). Monocyte‐related populations could also be located in bone marrow (BM), but studies correlating monocyte populations found in these two tissues are lacking. This study simultaneously analyzed PB and BM to phenotypically define multiple monocyte‐related subsets in each location. Methods: Murine PB and BM cells were simultaneously stained for monocyte‐related populations, using five‐color flow cytometry. Relevant subsets were defined on the basis of Ly‐6C, CD11b, and wheat germ agglutinin phenotype in addition to light‐scatter characteristics. These populations were extensively characterized for the expression of other myeloid and dendritic cell markers, adhesion molecules, chemokine receptors, and growth factor receptors. Results: Six monocyte‐related populations were defined, three each in BM and PB. No identical populations were found between the two tissues. Two BM populations and one PB population have heterogeneous expression of many markers, suggesting additional complexity among monocyte‐related subsets. Conclusions: The murine monocytic series comprises multiple subsets, differing between PB and BM. This study defines and extensively phenotypes six of these populations, providing preliminary information about possible developmental relationships and migratory capacities of these cells. © 2006 International Society for Analytical Cytology