Premium
E‐LDL and Ox‐LDL differentially regulate ceramide and cholesterol raft microdomains in human Macrophages
Author(s) -
Grandl Margot,
Bared Salim Maa,
Liebisch Gerhard,
Werner Tobias,
Barlage Stefan,
Schmitz Gerd
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20232
Subject(s) - ceramide , lipid raft , lactosylceramide , foam cell , microbiology and biotechnology , cholesterol , macrophage , chemistry , flow cytometry , sphingomyelin , lipid microdomain , confocal microscopy , low density lipoprotein , apoptosis , biochemistry , lipoprotein , biology , glycolipid , membrane , in vitro
Atherosclerosis is characterized by the generation of lipid‐loaded macrophage‐derived foam cells. To study the effect of different types of atherogenic lipoproteins, human macrophages were loaded with enzymatically degraded low density lipoprotein (E‐LDL) or oxidized LDL (Ox‐LDL). Cellular cholesterol content was increased by E‐LDL, whereas Ox‐LDL increased the ceramide content. Cell surface expression analysis by flow cytometry and confocal microscopy revealed that Ox‐LDL increased ceramide and lactosylceramide expression compared to E‐LDL loading and induced ceramide rafts, whereas loading with E‐LDL induced cholesterol‐rich microdomains. Formation of different rafts may have consequences for raft associated signaling in cholesterol homeostasis and apoptosis in human macrophages. © 2006 International Society for Analytical Cytology