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Multiplex analysis of cytokines in exhaled breath condensate
Author(s) -
Sack Ulrich,
Scheibe Robert,
Wötzel Michael,
Hammerschmidt Stefan,
Kuhn Hartmut,
Emmrich Frank,
Hoheisel Gerard,
Wirtz Hubert,
Gessner Christian
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20231
Subject(s) - exhaled breath condensate , multiplex , cytokine , medicine , breath gas analysis , immunology , lung , tumor necrosis factor alpha , proinflammatory cytokine , inflammation , biology , bioinformatics , asthma , anatomy
Abstract Background To improve monitoring of lung diseases, we analyzed cytokines in exhaled breath condensate (EBC). The main challenge in measurement of cytokines in EBC is the low protein content, which requires concentration steps that conflict with the need for excessive fluid required by most commonly used kits. Methods Here, a multiplex bead array for the detection of interleukins (IL) ‐1β, ‐6, ‐8, ‐10, TNF‐α, and IL‐12p70 was modified and validated for analysis in EBC samples. Furthermore, 33 healthy volunteers and 11 patients with acute lung injury were investigated. Results In patients with inflammatory lung diseases, cytokine levels for all investigated cytokines were higher in comparison to healthy smokers or healthy volunteers. Discussion Multiplexed immunoassays in highly sensitive approaches allow for cytokine detection in EBC. We found significant differences between patients and controls for all investigated cytokines. © 2005 International Society for Analytical Cytology