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Laser scanning cytometry in human brain slices
Author(s) -
Mosch Birgit,
Mittag Anja,
Lenz Dominik,
Arendt Thomas,
Tárnok Attila
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20228
Subject(s) - entorhinal cortex , human brain , cytometry , flow cytometry , immunohistochemistry , fluorescence , cortex (anatomy) , biology , pathology , microbiology and biotechnology , neuroscience , hippocampus , medicine , physics , optics
Background The Laser Scanning Cytometry (LSC) offers quantitative fluorescence analysis of cell suspensions and tissue sections. Methods We adapted this technique to immunohistochemical labelled human brain slices. Results We were able to identify neurons according to their labelling and to display morphological structures such as the lamination of the entorhinal cortex. Further, we were able to distinguish between neurons with and without cyclin B1 expression and we could assign the expression of cyclin B1 to the cell islands of layer II and the pyramidal neurons of layer V of the entorhinal cortex in Alzheimer's disease effected brain. In addition, we developed a method depicting the three‐dimensional distribution of the cells in intact tissue sections. Conclusions In this pilot experiments we could demonstrate the power of the LSC for the analysis of human brain sections. © 2006 International Society for Analytical Cytology