Iterative restaining as a pivotal tool for n‐color immunophenotyping by slide‐based cytometry | Zendy

Laffers Wiebke | Zendy; Mittag Anja | Zendy; Lenz Dominik | Zendy; Tárnok Attila | Zendy; Gerstner Andreas O. H. | Zendy

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Iterative restaining as a pivotal tool for n‐color immunophenotyping by slide‐based cytometry

Author(s) -

Laffers Wiebke,

Mittag Anja,

Lenz Dominik,

Tárnok Attila,

Gerstner Andreas O. H.

Publication year - 2006

Publication title -

cytometry part a

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.316

H-Index - 90

eISSN - 1552-4930

pISSN - 1552-4922

DOI - 10.1002/cyto.a.20216

Subject(s) - immunophenotyping , cytometry , flow cytometry , peripheral blood , pathology , computer science , medicine , immunology

Aim Slide‐based cytometry (SBC) allows to “ask a cell a second time.” We used this tool for detailed immunophenotyping of peripheral blood leukocytes (PBLs). Methods PBLs primarily stained for CD‐markers and DNA were immobilized on a glass slide and analyzed by laser scanning cytometry. Then, iterative restaining was applied for a second and a third analysis. Based on the cells' fixed location, analyses were merged on a single‐cell level. Results We analyzed six virtual immunostainings by “recycling” the PE‐channel for four CD‐markers. Conclusion Iterative restaining might prove to be a pivotal tool for n‐color immunophenotyping exclusive to SBC concepts. © 2006 International Society for Analytical Cytology

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