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Cytokine analysis to predict immunosuppression
Author(s) -
Barten Markus J.,
Rahmel Axel,
Bocsi József,
Boldt Andreas,
Garbade Jens,
Dhein Stefan,
Mohr Friedrich W.,
Gummert Jan F.
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20215
Subject(s) - immunosuppression , cytokine , multiplex , whole blood , dosing , medicine , immunology , pharmacology , biology , bioinformatics
Background Recently, it has been realized that TH1/TH2 cytokine production offer the unique possibility to predict drug efficacy. However, there is still an incessant need to explore assay conditions and techniques of analyzing cytokines, which are specific and reliable for monitoring drug efficacy. Methods In this study we used the multiplex bead array technique to detect cytokines of TH1/TH2 cells in whole blood of heart transplanted (HTx) recipients. Results We found significantly different levels of cytokine expression in HTx recipients compared with cytokine levels in patients prior to HTx. Furthermore, particular cytokine levels were significantly decreased 2 h after drug dosing, compared with cytokine levels before dosing in mitogen‐stimulated whole blood. Conclusions Cytokine analysis with the multiplex array technique in mitogen‐stimulated whole blood provides the possibility to predict immunosuppression. © 2006 International Society for Analytical Cytology