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A miniaturized wide‐angle 2D cytometer
Author(s) -
Singh Kirat,
Su Xuantao,
Liu Caigen,
Capjack Clarence,
Rozmus Wojciech,
Backhouse Christopher James
Publication year - 2006
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20199
Subject(s) - waveguide , scattering , microfluidics , light scattering , optics , microchannel , materials science , lens (geology) , lab on a chip , coupling (piping) , prism , optoelectronics , physics , nanotechnology , metallurgy
Background: We present an optical waveguide based cytometer that is capable of simultaneously collecting the light scattered by cells over a wide range of solid angles. Such comprehensive scattering data are a prerequisite for the microstructural characterization of cells. Methods: We use latex beads as cell mimics, and demonstrate the ability of this new cytometer to collect back‐scattered light in two dimensions (2D). This cytometer is based on a liquid‐core optical waveguide, excited by prism coupling, that also serves as the microfluidic channel. In principle, our use of a hemispherical lens allows the collection of scattered light from 0 to 180° in 2D. Results: The experimentally observed positions of the intensity peaks of the back‐scattered light agree well with theoretical prediction of scattering from both 4.0‐ and 9.6‐μm diameter latex beads. The position of the bead, relative to the axes of the hemispherical lens and the microchannel, strongly affects the scattering pattern. We discuss a computational method for determining these offsets. Conclusions: We show that wide‐angle 2D light scattering patterns of cell‐sized latex beads can be observed in a microfluidic‐based optical cytometer that uses leaky waveguide mode excitation. This chip‐based system is compatible with emerging chip‐based technologies. © 2005 Wiley‐Liss, Inc.