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Normalization of multicolor fluorescence in situ hybridization (M‐FISH) images for improving color karyotyping
Author(s) -
Wang YuPing,
Castleman Kenneth R.
Publication year - 2005
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20116
Subject(s) - normalization (sociology) , artificial intelligence , computer science , pixel , pattern recognition (psychology) , classifier (uml) , fluorescence in situ hybridization , computer vision , chromosome , biology , genetics , sociology , anthropology , gene
Multiplex or multicolor fluorescence in situ hybridization (M-FISH) is a recently developed cytogenetic technique for cancer diagnosis and research on genetic disorders. By simultaneously viewing the multiply labeled specimens in different color channels, M-FISH facilitates the detection of subtle chromosomal aberrations. The success of this technique largely depends on the accuracy of pixel classification (color karyotyping). Improvements in classifier performance would allow the elucidation of more complex and more subtle chromosomal rearrangements. Normalization of M-FISH images has a significant effect on the accuracy of classification. In particular, misalignment or misregistration across multiple channels seriously affects classification accuracy. Image normalization, including automated registration, must be done before pixel classification.