Premium
Absence of cross‐reactivity between murine Ly‐6C and Ly‐6G
Author(s) -
Nagendra Sanjai,
Schlueter Annette J.
Publication year - 2004
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.20007
Subject(s) - reactivity (psychology) , chemistry , computational biology , biology , biophysics , medicine , pathology , alternative medicine
Background The Ly‐6 family has many members, including Ly‐6C and Ly‐6G. A previous study suggested that the anti‐Ly‐6G antibody, RB6‐8C5, may react with Ly‐6C hi murine bone marrow (BM) cells. This finding has been interpreted as cross‐reactivity of RB6‐8C5 with the Ly‐6C antigen, and has been generalized to many hematopoietic cell types, using the terminology Ly‐6G/C. The present study was undertaken to determine whether anti‐Ly‐6G antibodies truly cross‐react with the Ly‐6C antigen on multiple hematopoietic cell types. Methods Splenocytes, thymocytes, and BM cells obtained from Ly‐6.1 and Ly‐6.2 strains of mice were stained with a variety of antibodies to Ly‐6C and Ly‐6G. Flow cytometric analysis was performed on these populations. Results Evaluation of anti‐Ly‐6C and anti‐Ly‐6G staining showed only Ly‐6C expression and no Ly‐6G expression on subsets of splenic T and B cells and thymocytes from Ly‐6.1 and Ly‐6.2 mice. Bone marrow cells were identified that express both Ly‐6G and Ly‐6C; no Ly‐6G + Ly‐6C − populations were seen. Conclusions Multiple Ly‐6C + hematopoietic cell populations were identified that do not stain with anti‐Ly‐6G antibodies. This calls into question the use of the Ly‐6G/C nomenclature and suggests that epitopes recognized by anti‐Ly‐6G antibodies should simply be designated Ly‐6G. © 2004 Wiley‐Liss, Inc.