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Analysis of P‐glycoprotein–mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay
Author(s) -
Park Suk W.,
Lomri Noureddine,
Simeoni Luiz A.,
Fruehauf John P.,
Mechetner Eugene
Publication year - 2003
Publication title -
cytometry part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.316
H-Index - 90
eISSN - 1552-4930
pISSN - 1552-4922
DOI - 10.1002/cyto.a.10039
Subject(s) - p glycoprotein , immunostaining , microbiology and biotechnology , efflux , biology , monoclonal antibody , chemistry , biochemistry , antibody , immunology , immunohistochemistry , multiple drug resistance , antibiotics
Background During transport‐associated adenosine triphosphate hydrolysis, P‐glycoprotein (Pgp) undergoes conformation transitions detected by UIC2, a functional anti‐Pgp monoclonal antibody. A newly developed UIC2 shift assay is based on increased UIC2 reactivity in the presence of Pgp substrates. All peripheral blood leukocytes express low Pgp levels. The existing antibody‐based detection methods are limited in their sensitivity and require additional techniques to simultaneously analyze Pgp expression and efflux, making it difficult to ascertain the physiologic role of Pgp‐mediated transport. Methods We validated the UIC2 shift assay against UIC2 immunostaining and DiOC 2 efflux. The UIC2 shift assay was then used to characterize Pgp functional expression and its physiologic substrates in peripheral blood leukocytes. Results A strong correlation was observed between the UIC2 shift assay versus immunostaining and dye efflux tests. The UIC2 shift assay showed improved sensitivity (compared with conventional UIC2 staining) and allowed for simultaneous detection of Pgp expression and function. Using this assay, we identified several new Pgp substrates, including monensin and retinol, and confirmed that interleukin‐2 and interferon‐γ can be transported by Pgp. Conclusions Our findings validate the use of the UIC2 shift assay in MDR1 detection and support the idea that Pgp plays a physiologic role in immunoregulation. Cytometry Part A 53A:67–78, 2003. © 2003 Wiley‐Liss, Inc.