Open AccessNear‐IR dyes in three‐color volumetric capillary cytometry: Cell analysis with 633‐ and 785‐nm laser excitation
Author(s) -
Lee Linda G.,
Woo Sam L.,
Head David F.,
Dubrow Robert S.,
Baer Thomas M.
Publication year - 1995
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990210203
Subject(s) - fluorescence , cytometry , laser , dye laser , chemistry , materials science , laser scanning , analytical chemistry (journal) , flow cytometry , optics , chromatography , microbiology and biotechnology , biology , physics
Several fluorescent dyes that absorb in the nearinfrared are described. The photostability and aggregation properties of the dyes were examined. Two of the dyes, BHMP and BHDMAP, emit at 805 nm and were useful dyes for protein labeling. A dual‐laser, three‐color scanning instrument was constructed. CD3 + CD4 + and CD3 + CD8 + populations were enumerated in undiluted, whole blood based on the fluorescence of Cys5, Cy5.5 and BHMP. © 1995 Wiley‐Liss, Inc.