Open AccessFlow cytometric determination of aminopeptidase activities in viable cells using fluorogenic rhodamine 110 substrates
Author(s) -
Ganesh S.,
Klingel S.,
Kahle H.,
Valet G.
Publication year - 1995
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990200409
Subject(s) - exopeptidase , endopeptidase , aminopeptidase , intracellular , biochemistry , cleavage (geology) , flow cytometry , biology , proteolysis , amino acid , substrate (aquarium) , peptide , cleave , chemistry , microbiology and biotechnology , enzyme , leucine , paleontology , ecology , fracture (geology)
Aminopeptidases (AP) are ubiquitously occuring, nonspecific exopeptidases involved in protein degradation. They cleave the N‐terminal amino acid of peptides and occur in practically all mammalian cells and tissues. Physiological and pathological processes such as metastasis of tumors and inflammation have been thought to involve changes in AP activities. Determination of AP (EC 3.4.11.X) activity in viable cells by flow cytometry was the subject of this study because of its general biological and clinical interest. Different bis‐substituted rhodamine 110 (R110) peptide derivatives were synthesised and used as AP‐ and exopeptidase (EC 3.4.13.X–EC 3.4.14.X) substrates for flow cytometric measurements. Intracellular AP activities in viable lympho‐, mono‐, granulo‐, and thrombocytes were detected by fluorescence increase from R110 following intracellular substrate cleavage. Eukaryotic‐AP do not cleave D‐aminoacids and hence NH 2 (D‐Leu) 2 R110 substrate served as negative control. Specific substrate cleavage by AP is shown by complete inhibition of fluorescence generation following preincubation of cells with leucinechloromethylketone inhibitor. R110 AP‐ and exopeptidase substrates are suitable indicators for coupled endopeptidase reactions due to their rapid cleavage and largely pH independent generation of intracellular fluorescence. © 1995 Wiley‐Liss, Inc.