Open AccessEnumeration of viable Candida albicans blastospores using tetrabromofluorescein (Eosin Y) and flow cytometry
Author(s) -
Costantino Paul J.,
Budd Dianne E.,
Gare Norman F.
Publication year - 1995
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990190413
Subject(s) - enumeration , flow cytometry , candida albicans , staining , microbiology and biotechnology , biology , eosin , chemistry , mathematics , genetics , combinatorics
A rapid assay was developed for determination of the viability of blastospores of Candida albicans utilizing flow cytometry to detect the accumulation of tetrabromofluorescein in non‐viable yeast cells. Eosin Y was shown to stain non‐viable C. albicans blastospores selectively without affecting the cellular viability of competent yeast cells or resulting in non‐specific staining of viable cells. Results of these studies show that this flow cytometric method of determining cell viability of C. albicans is more accurate and more precise than the more common method of enumerating colony forming units. © 1995 Wiley‐Liss, Inc.