Open AccessThe macro flow planktometer: A new device for volume and fluorescence analysis of macro plankton including triggered video imaging in flow
Author(s) -
Hüller Reinhold,
Glossner Ewald,
Schaub Stefan,
Weingärtner Johann,
Kachel Volker
Publication year - 1994
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990170202
Subject(s) - macro , computer science , volume (thermodynamics) , modular design , data acquisition , volumetric flow rate , flow measurement , software , flow (mathematics) , computer hardware , process engineering , biological system , physics , engineering , quantum mechanics , mechanics , thermodynamics , programming language , operating system , biology
A newly developed macro flow planktometer is described for measuring plankton organisms with a size of 100–2,000 μm by the flow principle in time‐saving and noncontact fashion. Based on the method of changes in electric resistance (Coulter principle), the device allows determination of the body volume of living organisms in their natural medium (fresh or seawater) at a measuring rate of up to 50 organisms per second. Simultaneous, laser‐excited fluorescence measurement permits quantitative detection of fluorescent substances within the organisms. Controlled by the multiparametric measurement, video images of organisms of particular interest can be taken in the flow chamber using a stroboscopic imaging system. Valuable morphological information on the material under test is thus available in addition to the analytical measurement. Data acquisition and imaging, the total system control, and the fast data evaluation are performed with an IBM‐AT compatible computer using an extensive software package. The easy‐to‐handle desktop unit can be used both in land laboratories and on ships. The modular structure of the system permits any desired combination of individual components to adapt it to various requirements. The operability of this new measuring system is demonstrated by several applications. © 1994 Wiley‐Liss, Inc.