Open AccessFlow cytometric evaluation of human basophils
Author(s) -
Gane Pierre,
Pecquet Catherine,
Lambin Patrick,
Abuaf Nissen,
Leynadier Francisque,
Rouger Philippe
Publication year - 1993
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990140316
Subject(s) - immunoglobulin e , flow cytometry , microbiology and biotechnology , antigen , antibody , staining , chemistry , cell , biology , immunology , biochemistry , genetics
The expression of CD45 and IgE cell surface antigens on human leucocytes was studied by flow cytometry. More than 80% of sorted cells that expressed low CD45 (CD45 dim ) and high IgE (IgE bright ) antigen site density were identified as basophils. Immunomagnetic depletion of the CD45 dim ‐IgE bright cell subset by a biotin‐coupled anti‐IgE antibody and streptavidin‐coated magnetic beads was greater than 90%, and more than 80% of cells binding significant numbers of beads exhibited the morphological characteristics of basophils. Interestingly, when the cell staining was performed in the presence of calcium and magnesium, we observed a significant increase of CD45 and an equivalent decrease of IgE cell surface expression, as well as an IgE concentration dependent diminution of the number of CD45 dim ‐IgE bright cells. © 1993 Wiley‐Liss, Inc.