Open AccessFlow injection cytometry: A new approach for sample and solution handling in flow cytometry
Author(s) -
Lindberg Walter,
Ruzicka Jaromir,
Christian Gary D.
Publication year - 1993
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990140217
Subject(s) - flow cytometry , flow (mathematics) , cytometry , sample (material) , chemistry , chromatography , environmental science , biology , mechanics , microbiology and biotechnology , physics
A prototype instrument for sample and solution handling in flow cytometry has been constructed. The system is modular and allows the control of any combination of up to 4 pumps, 2 selection valves, and 2 injection valves. These devices are controlled by a computer using TTL‐logic. The flow injection instrument is interfaced to the flow cytometer via a 6‐port injection valve, thereby facilitating virtually any flow pattern to be implemented without affecting the fluidics of the cytometer. Salient features of the instrument are accurate control of the volume of injected sample and reagent, reproducible timing, and controlled mixing conditions. Results from model experiments of on‐line staining of trout erythrocytes with different concentrations of 4′,6‐diamidino‐2‐phenylindole (DAPI) and propidium iodide (PI) are shown. Possible ways to improve the performance and utility of the instrument are also discussed. © 1993 Wiley‐Liss, Inc.