Open AccessRhodamine 123 fluorescence of immortal hybridoma cell lines as a function of glucose concentration
Author(s) -
Borth Nicole,
Kral Gerda,
Katinger Hermann
Publication year - 1993
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990140112
Subject(s) - rhodamine 123 , fluorescence , glutamine , cell culture , biochemistry , biology , rhodamine , glycolysis , glucose uptake , biophysics , chemistry , metabolism , microbiology and biotechnology , endocrinology , genetics , amino acid , insulin , physics , quantum mechanics , multiple drug resistance , antibiotics
The fluorescence of rhodamine 123 stained cells has been described to specifically reflect the activity of mitochondria. Changes in the intensity of fluorescence observed in stimulated lymphocytes were attributed to an increased glycolytic activity of cells due to increased growth rates. Previously reported changes in mitochondrial activity observed in batch cultures were likewise attributed to changed growth rates. In this study we report that the Rh123 fluorescence of hybridoma cell lines in batch culture more closely correlates to the glucose concentration in the culture supernatant than to growth rates. When cells are transferred into glutamine free medium with defined glucose concentrations ranging from 0 to 3,000 mg/L the mean Rh123 fluorescence adapts to the respective glucose concentration within 6 hours and gives a linear correlation. This can be explained by the previously described dependence of specific glucose consumption rates on glucose availability in the medium. The importance of controlling glucose availability, especially in large scale fermentations, is discussed. © 1993 Wiley‐Liss, Inc.