Open AccessMeasuring percent lymphocytes by flow cytometry to calculate absolute lymphocyte subset counts for HIV + specimens
Author(s) -
Gale Howard B.,
Henry Keith
Publication year - 1992
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990130211
Subject(s) - hematology analyzer , flow cytometry , lymphocyte , hematology , resolution (logic) , sample (material) , human immunodeficiency virus (hiv) , immunology , cell counting , biology , cytometry , cell , pathology , medicine , chromatography , chemistry , genetics , computer science , artificial intelligence , cell cycle
A comparison was made of lymphocyte percentages from an automated hematology analyzer (ELT 15) vs. a fluorescence flow cytometer (Cytofluorograf). The hematology values were consistently higher than the flow (by greater than 10% for 13 of 50 HIV + specimens). The findings were similar to three other pairings: H * 1 vs. Cytofluorograf, Cell Dyn 3000 vs. Cytofluorograf, and Cell Dyn 3000 vs. EPICS Profile. In another comparison manual percent lymphocytes matched much better with flow values. Factors of sample preparation and instrument analysis as they relate to nonrandom cell loss and lymphocyte resolution are examined.