Open AccessRecovery of RNA from flow‐sorted fixed cells
Author(s) -
Khochbin Saadi,
Grunwald Didier,
Pabion Michel,
Lawrence JeanJacques
Publication year - 1990
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990110803
Subject(s) - flow cytometry , rna , cell cycle , cell sorting , cell , sorting , microbiology and biotechnology , computational biology , biology , computer science , gene , genetics , algorithm
We describe a method for the preparation of RNA from ethanol‐fixed cells, allowing analysis of the RNA from cells “frozen” in a given physiological state. This technique may have important applications in experiments which require prolonged cell manipulations beforre RNA preparation, such as investigations of cell‐cycle‐regulated gene exprression, which require the preparation of cells for cell‐cycle flow analysis, and even for long‐term cell sorting. It eliminates all the inconveniences associated with the use of fresh cells, and allows cell‐cycle biologists to couple flow cytometry methodology with the advancing techniques of molecular biology.