Open AccessEffect of focus on cell detection and recognition by the cell analyzer
Author(s) -
Spadinger Ingrid,
Poon Steven S. S.,
Palcic Branko
Publication year - 1990
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990110403
Subject(s) - focus (optics) , spectrum analyzer , cytometry , position (finance) , signal (programming language) , computer science , microscopy , artificial intelligence , biomedical engineering , optics , computer vision , cell , biological system , materials science , physics , biology , medicine , finance , economics , genetics , programming language
The effect of defocusing on the quality of signals from live cells detected by an automated image cytometry device, the Cell Analyzer, was examined. The influence of these effects on the ability of this device to automatically locate cells plated into a tissue culture flask was then determined by measuring the performance of cell detection and recognition procedures as a function of focus setting. Acceptable limits for deviation from the optimal focus setting (as determined by microscope objective position) were found to be similar for both these procedures, ranging from 40 μm below to 25 μm above the optimal focus position. These limits were asymmetrical about ideal focus due to a pronounced asymmetry in the effects of positive and negative defocusing on the cell signal.