Open AccessA spectrophotometric method for determining the stream sample core diameter of a flow cytometer
Author(s) -
Blume Philip
Publication year - 1989
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990100316
Subject(s) - sample (material) , chromatography , core (optical fiber) , flow (mathematics) , chemistry , computer science , physics , telecommunications , mechanics
A simple, rapid, and accurate method is described for the determination of flow cytometer stream sample core diameters. A precisely formulated solution of potassium dichromate is introduced as a sample, the stream is collected, and its absorbance is measured using a spectrophotometer. This permits the calculation of the factor by which the original solution was diluted and, given the stream diameter, the sample core diameter.