Open AccessA digital image microscopy system for rare‐event detection using fluorescent probes
Author(s) -
Lee Benjamin R.,
Haseman David B.,
Reynolds C. Patrick
Publication year - 1989
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990100304
Subject(s) - bone marrow , computer science , pathology , fluorescence microscope , biomedical engineering , fluorescence , biology , computer vision , medicine , physics , optics
Instrumentation for rare‐event analysis should be capable of reliably detecting infrequent cells (< 1:10,000) while both excluding false‐positive signals and including true positive cells found in multicell clumps. We have developed a digital image microscopy (DIM) system in which a cytospin of 2 million cells is scanned with an intensified video camera (ISIT) using an IBM PC AT microcomputer‐controlled microscope stage. PASCAL software controls the stage and analyzes video input, storing the location of positive cells to magnetic disk. The user can then “replay” each positive cell under computer control for either visual confirmation or analysis using other fluorescent probes. The computer requires 24 min to scan a cytoprep of 2 million cells, while playback for visual confirmation by the user averages 5 min. Using Hoechst–33342 premarked cells seeded into bone marrow as a model system, we found that the DIM system reliably detects one target cell per million marrow cells. With appropriate immunological markers, this system will aid in evaluating bone marrow purged of tumor cells prior to transplantation and should also be useful for detection of minimal residual disease in blood or bone marrow from patients with leukemia or solid tumors.