Open AccessApplication of bromodeoxyuridine incorporation measurements to the determination of cell distribution within the S phase of the cell cycle
Author(s) -
Khochbin Saadi,
Chabanas Agnés,
Albert Philippe,
Albert Jacques,
Lawrence JeanJacques
Publication year - 1988
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990090516
Subject(s) - bromodeoxyuridine , phase (matter) , cell cycle , chemistry , cell , computer science , materials science , cell growth , biochemistry , organic chemistry
Flow cytofluorimetric measurement of incorporated bromodeoxyuridine, using a double‐stained cell population, allows the determination of the distribution of cells along the cell cycle (1). We have developed a simple computer program for the direct treatment of 64 × 64 channel histograms. This analysis appears to provide interesting data about the distribution of cells in the various phases of the cell cycle, namely the S phase. Two examples have been chosen to illustrate possible fields for the application of such a program. Comparison of two cell lines such as friend murine erythroleukemia cells (MELC) and fibroblasts FR3T3 cells has shown that this analysis can be used for cell‐cycle characterization of a given cell line. The program also allows the differential analysis of cell distribution along the cell cycle as a function of a given parameter. This possibility has been applied to study the variation of cell‐cycle parameters as a function of the time of induced differentiation of MELC and reveals changes in the distribution of the cells along the various phases of the cell cycle, namely in the S phase.