Open AccessImproved program for the analysis of DNA histograms
Author(s) -
Ormerod M. G.,
Payne A. W. R.,
Watson J. V.
Publication year - 1987
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990080617
Subject(s) - histogram , pascal (unit) , computer science , set (abstract data type) , bromodeoxyuridine , dna , algorithm , computer program , pattern recognition (psychology) , chemistry , artificial intelligence , programming language , biochemistry , cell growth , image (mathematics)
We describe a computer program which will rapidly analyze a set of DNA histograms without further operator intervention after the initial input. The algorithm used was modified from that of Watson et al. ( Cytometry 8:1–8, 1987 ). The program, coded in Pascal, has been implemented on two different systems. It has been tested by using simulated data and experimental data from cells labelled with bromodeoxyuridine. The program has been in use in this and other laboratories for over two years and has proved to be robust and fast and to give a reasonable approximation to the experimental data. It is particularly useful when dealing with badly perturbed DNA histograms.