Open AccessElectron microscopy microsampling of isolated nuclei sorted by flow cytometry
Author(s) -
Zini Nicoletta,
Matteucci Alessandro,
Squarzoni Stefano,
Galanzi Angela,
Rizzoli Riccardo,
Papa Stefano
Publication year - 1986
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990070617
Subject(s) - sorting , diafiltration , flow cytometry , electron microscope , chemistry , analytical chemistry (journal) , chromatography , materials science , biophysics , computer science , physics , biology , membrane , microbiology and biotechnology , optics , biochemistry , algorithm , microfiltration
This paper describes an efficient method to concentrate for electron microscopic examination minute quantities of subcellular particles obtained by cytofluorimetric sorting. The advantages of this micromethod, based on diafiltration on Millipore filters under constant positive nitrogen pressure, are discussed.