Open AccessSequential monitoring of peripheral blood lymphocyte subsets in rats
Author(s) -
Thompson Sandy C.,
Bowen Kerry M.,
Burton Robert C.
Publication year - 1986
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990070210
Subject(s) - lymphocyte subsets , enumeration , peripheral blood , flow cytometry , lymphocyte , immunology , biology , peripheral blood lymphocyte , t lymphocyte , computational biology , staining , computer science , t cell , mathematics , genetics , immune system , combinatorics
The methodology of enumeration of T cell subsets in humans is well established and has widespread clinical application. Sequential monitoring of lymphocyte subsets in small animals requires a consistent and reliable methodology for staining and enumerating lymphocyte populations. The optimal conditions for enumerating subclasses of rat peripheral blood lymphocytes with the Spectrum III flow cytometer are described. The sources of biological variation and technical error that were considered in optimizing the techniques and the limitations in applying them are discussed.