Open AccessFlow cytometric and radioisotopic determanations of platelet survival time in normal cats and feline leukemia virus‐infected cats
Author(s) -
Jacobs R. M.,
Boyce J. T.,
Kociba G. J.
Publication year - 1986
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990070109
Subject(s) - cats , platelet , flow cytometry , feline leukemia virus , fluorescein isothiocyanate , centrifugation , biology , fluorescein , andrology , immunology , pathology , chemistry , microbiology and biotechnology , medicine , biochemistry , physics , quantum mechanics , fluorescence
This study demonstrates the potential usefulness of a flow cytometric technique to measure platelet survival time in cats utilizing autologous platelets labeled in vitro with fluorescein isothiocyanate (FITC). When compared with a 51 Cr method, no significant differences in estimated survival times were found. Both the 51 Cr and FITC‐labeling procedures induced similar changes in platelet shape and collagen‐induced aggregation. Platelets labeled with FITC had significantly greater volumes compared with those of glutaraldehyde‐fixed platelets. These changes were primarily related to the platelet centrifugation and washing procedures rather than the labels themselves. This novel technique potentially has wide applicability to cell circulation time studies as flow cytometry equipment becomes more readily available. In a preliminary study of the platelet survival time in feline leukemia virus (FeLV)‐infected cats, two of three cats had significantly reduced survival times using both flow cytometric and radioisotopic methods. These data suggest increased platelet turnover in FeLV‐infected cats.