Open AccessDifferences between labeling index and DNA histograms in assessing s‐phase cells from a homogenous group of chronic phase CML patients
Author(s) -
Raza Azra,
Bhayana Ranjan,
Ucar Kalust,
Kirshner Jeffery,
Preisler Harvey D.
Publication year - 1985
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990060509
Subject(s) - histogram , aneuploidy , dna , chronic myelogenous leukemia , thymidine , chromosomal translocation , biology , microbiology and biotechnology , chromosome , leukemia , immunology , genetics , computer science , artificial intelligence , image (mathematics) , gene
Abstract The reliability of DNA histogram analysis in accurately estimating S‐phase cells from human tumors was tested by comparing the results to those of simultaneously obtained tritiated thymidine labeling index (LI) studies. Patients with chronic myelocytic leukemia (CML) during chronic phase were selected for study because the Philadelphia chromosome (Ph) was the only cytogenetic abnormality in each case and, since it is a balanced translocation, the frequently encountered problem of aneuploidy in human neoplastic cells was avoided. Unfortunately, when 30 CML patients were studied simultaneously by DNA histogram analysis and LI studies, the correlation coefficient between the two results was only r = 0.611. A comparison of three different mathematical programs for DNA histogram analysis showed that none was completely satisfactory. We conclude that DNA histogram analysis does not provide the same data as autoradiographically processed labeling index studies even in patients with Ph‐positive CML during the chronic phase when the situation is not complicated by additional aneuploidy.