Open AccessThe LLNL high‐speed sorter: Design features, operational characteristics, and biological utility
Author(s) -
Peters D.,
Branscomb E.,
Dean P.,
Merrill T.,
Pinkel D.,
Van Dilla M.,
Gray J. W.
Publication year - 1985
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990060404
Subject(s) - hiss , national laboratory , chinese hamster ovary cell , production rate , sort , computer science , environmental science , process engineering , biology , engineering , physics , engineering physics , nuclear physics , information retrieval , genetics , cell culture , electron
This report describes a dual‐beam, high‐speed sorter (HiSS) with a droplet production rate of 220,000 s −1 now in routine use at the Lawrence Livermore National Laboratory. The system can process and sort objects at rates in excess of 20,000 s −1 . We report here on the development of HiSS, describe its operational characteristics, and evaluate its utility for analysis and purification of human chromosomes, human erythrocytes, and living Chinese hamster ovary cells.