Open AccessInexpensive upgrading of a FACS I and isolation of rare somatic variants by double‐fluorescence sorting
Author(s) -
Weichel Walter,
Liesegang Bernhard,
Gehrke Klaus,
Göttlinger Christoph,
Holtkamp Bodo,
Radbruch Andreas,
Stackhouse Thomas K.,
Rajewsky Klaus
Publication year - 1985
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990060206
Subject(s) - fluorescence , monoclonal antibody , cell sorting , microbiology and biotechnology , immunofluorescence , biology , isolation (microbiology) , somatic cell , chemistry , flow cytometry , antibody , biochemistry , genetics , gene , optics , physics
We have modified a FACS I by addition of a tunable dye laser and an optical system for fluorescence detection that allows physically independent measurement of green and red immunofluorescence. These modifications are inexpensive and should be applicable to most single‐laser system. By using the modified machine and double‐fluorescence activated selection with H‐2K k ‐specific monoclonal antibodies labelled with FITC or Texas Red, we have isolated from a murine T‐cell lymphoma line variant subclones expressing structurally altered histocompatibility class I (H‐2K k ) molecules.