Open AccessPreparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry
Author(s) -
Kooi M. W.,
Aten J. A.,
Stap J.,
Kipp J. B. A.,
Barendsen G. W.
Publication year - 1984
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990050519
Subject(s) - flow cytometry , cytometry , microbiology and biotechnology , chemistry , biology , chromatography
Abstract A method is described by which metaphase chromosomes are isolated from cells of a transplantable rat sarcoma. The chromosomes are derived from cells in a suspension prepared by trypsinisation of tumours from rats that have been treated with vindesine 24 h before excision in order to accumulate cells in mitosis. Histograms obtained for the chromosomes of the solid tumour are compared with flow karyotypes of cells cultured for 20 h or for several generations in vitro.