Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry | Zendy

Kooi M. W. | Zendy; Aten J. A. | Zendy; Stap J. | Zendy; Kipp J. B. A. | Zendy; Barendsen G. W. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Preparation of chromosome suspensions from cells of a solid experimental tumour for measurement by flow cytometry

Author(s) -

Kooi M. W.,

Aten J. A.,

Stap J.,

Kipp J. B. A.,

Barendsen G. W.

Publication year - 1984

Publication title -

cytometry

Language(s) - English

Resource type - Journals

eISSN - 1097-0320

pISSN - 0196-4763

DOI - 10.1002/cyto.990050519

Subject(s) - flow cytometry , cytometry , microbiology and biotechnology , chemistry , biology , chromatography

A method is described by which metaphase chromosomes are isolated from cells of a transplantable rat sarcoma. The chromosomes are derived from cells in a suspension prepared by trypsinisation of tumours from rats that have been treated with vindesine 24 h before excision in order to accumulate cells in mitosis. Histograms obtained for the chromosomes of the solid tumour are compared with flow karyotypes of cells cultured for 20 h or for several generations in vitro.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research