Open AccessEffects of temperature on the yeast cell cycle analyzed by flow cytometry
Author(s) -
Vai Marco,
Vai Marina,
Frascotti Gianni
Publication year - 1984
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990050515
Subject(s) - yeast , saccharomyces cerevisiae , cell cycle , exponential growth , biology , budding yeast , flow cytometry , budding , biophysics , growth rate , dna replication , incubation , microbiology and biotechnology , cell , biochemistry , dna , genetics , physics , geometry , mathematics , quantum mechanics
Abstract The effects of temperature (in the range 15–36°C) on growth and the nuclear and budding cycle have been studied in populations of the yeast Saccharomyces cerevisiae exponentially growing in batch on yeast nitrogen base (YNB) glucose medium. The maximal rate of exponential growth is achieved at 30°C, and a transition point is apparent at about 20°C. At all tested temperatures DNA replication begins when cells are still unbudded and both the budded period and the postreplicative period have the same temperature dependence. A temperature compensatory mechanism seems to operate in S phase, during which duration remains relatively constant, in the range 21–36°C, while duration of G2+M phases shows a much more pronounced temperature dependence. The results are discussed in terms of a cell‐cycle model for budding yeast.