Open AccessThe application of perpendicular and forward light scatter to assess nuclear and cellular morphology
Author(s) -
Benson Mitchell C.,
McDougal Denise C.,
Coffey Donald S.
Publication year - 1984
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990050513
Subject(s) - perpendicular , optics , grading (engineering) , light scattering , physics , scattering , biology , mathematics , geometry , ecology
Perpendicular and forward light scatter have been employed in a multiparametric approach to distinguish the various lines of the transplantable Dunning R3327 rat prostatic adenocarcinoma. Perpendicular light scatter has been shown to correlate with nuclear size and shape. The average intensity of forward light scatter and perpendicular light scatter signals was demonstrated to increase as cells became less well‐differentiated. The combination of perpendicular and forward light scatter allows for the discrimination of histologically indistinguishable tumors and may therefore be useful to establish a system of grading cancer.