Open AccessThe relationship between thrombomodulin expression and cell cycle stages in cultured rabbit endothelial cells
Author(s) -
Debault Lawrence E.,
Esmon Naomi L.,
Esmon Charles T.
Publication year - 1984
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990050415
Subject(s) - immunofluorescence , thrombomodulin , cell cycle , propidium iodide , cytoplasm , cell , biology , intracellular , microbiology and biotechnology , antigen , flow cytometry , golgi apparatus , fluorescence , antibody , biochemistry , immunology , apoptosis , platelet , thrombin , physics , quantum mechanics , programmed cell death
Cultured rabbit endothelial cells have significant but variable amounts of thrombomodulin (TM), both on their surface as well as inside the cell. To determine if variations in TM antigen is cell cycle related, cells with very high levels of TINI antigen were identified and staged according to the intracellular distribution and relative amounts of the antigen, using immunofluorescence techniques. After staging, the nuclear DNA content of each of these cells was determined by measuring the propidium iodide (PI) fluorescence intensity cytophotometrically. Stages 1, 2, and 3, which exhibited TM immunofluorescence in the golgi area, clustered to the G1 phase of the cell cycle. Cells without discernible golgi fluorescence (stages 4 and 5) but with variable amounts of cytoplasmic and surface fluorescence appeared to have little or no relationship to the cell cycle.