Open Access7‐amino‐actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry
Author(s) -
Zelenin Alexander V.,
Poletaev Andrey I.,
Stepanova Natalija G.,
Barsky Viktor E.,
Kolesnikov Viktor A.,
Nikitin Sergei M.,
Zhuze Alexey L.,
Gnutchev Nikolai V.
Publication year - 1984
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990050410
Subject(s) - flow cytometry , dna , cytometry , staining , fluorophore , microbiology and biotechnology , stain , fluorescence , biology , chemistry , biochemistry , optics , genetics , physics
A technique for DNA amount determination by flow cytometry based on the use of 7‐amino‐actinomycin D (7‐amino‐AMD), a fluorescent analogue of antibiotic actinomycin has been investigated, and a particular staining procedure has been developed. The procedure includes short fixation in 70% ethanol and staining for 20 min in 10‐5M solution of 7‐amino‐AMD at pH7. The results of DNA content measurements are very reproducible. The histograms obtained have a coefficient of variation less than 3%. The absorption maximum of the complex of 7‐amino‐AMD with DNA is situated in the green spectrum region, making this stain particularly suitable for argon laser flow cytometry.