Open AccessA differential amplifier circuit for reducing noise in axial light loss measurements
Author(s) -
Steinkamp John A.
Publication year - 1983
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990040112
Subject(s) - oscilloscope , photodiode , amplifier , optics , materials science , differential amplifier , detector , noise (video) , signal (programming language) , laser , optoelectronics , physics , computer science , cmos , artificial intelligence , image (mathematics) , programming language
Simplified electronics have been developed for reducing laser excitation source light intensity fluctuations in flow cytometric axial light loss (extinction) measurements. By continuously monitoring the laser output with a photodiode detector and combining it with the axial light loss signal from a second detector using a high‐speed differential amplifier, background interference is reduced 10 to 40 dB. Oscilloscope waveforms and frequency distribution histograms recorded from uniform‐size polystyrene latex spheres and human mononuclear blood cells are used to illustrate the noise reduction capabilities.