Open AccessCell surface antigens reacting with antiretrovirus sera on normal mouse spleen cells: A flow cytometric study
Author(s) -
Szabó Gábor,
Dtóth Ferenc,
Réthy Antal
Publication year - 1983
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990040107
Subject(s) - immunofluorescence , flow cytometry , antigen , microbiology and biotechnology , spleen , biology , antiserum , cell cycle , antibody , cell , leukemia , chemistry , immunology , biochemistry
The development of erythroleukemia in Balb/c mice injected with Rauscher leukemia virus has been followed by indirect immunofluorescence technique and flow cytometry, using antiserum against disrupted virions. The progression of the disease was accompanied by a great increase in the number of large, immunofluorescence positive cells. A subpopulation of normal spleen cells was also highly positive. The expression of the antigens in normal cells was examined in relation to the cell cycle. The majority of the S‐G2/M phase cells were found in the antigen positive compartment of larger cells. A two‐color analysis of immunofluorescence and DNA content revealed that the distribution of antigen expression in G1 phase was broad, gradually decreasing from a low‐intensity mode. The cell with double DNA content distributed evenly around a modus of five‐fold higher intensity. In experiments with stimulated bonemarrow cells, superiority of S‐phase cells in anti‐Rauscher serum binding was found. Cell‐surface gp70 antigen is suggested to be involved in this cell‐cycle dependent binding of antibodies by normal cells.