Open AccessDirect determination of cell diameter, surface area, and volume with an electronic volume sensing flow cytometer
Author(s) -
Schwartz A.,
Sugg H.,
Ritter T. W.,
FernandezRepollet E.
Publication year - 1983
Publication title -
cytometry
Language(s) - English
Resource type - Journals
eISSN - 1097-0320
pISSN - 0196-4763
DOI - 10.1002/cyto.990030613
Subject(s) - volume (thermodynamics) , flow (mathematics) , calibration , materials science , surface (topology) , biomedical engineering , analytical chemistry (journal) , chemistry , chromatography , physics , mechanics , mathematics , geometry , engineering , thermodynamics , quantum mechanics
A method is described in which the volume, surface area, and diameter of spherical cells can be quickly and easily determined from linear calibration curves using a flow cytometer which measures electronic volume and is equipped with a log amplifier.